Composite

Part:BBa_K3120016:Design

Designed by: Ruoyi Xue   Group: iGEM19_BM-AMU   (2019-08-14)


pU6+gRNA2+pCAG+hspCas9+AmpR promoter+AmpR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1202
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2029
    Illegal NgoMIV site found at 3133
    Illegal NgoMIV site found at 3206
    Illegal NgoMIV site found at 3691
    Illegal NgoMIV site found at 4600
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 5890


Design Notes

Source

References